Supplementation of rapamycin during in vitro maturation promotes oocyte quality and subsequent embryo development in bovine

Abstract Background The developmental competence of oocyte derived from in vitro maturation (IVM) is significantly lower than those matured vivo. Rapamycin an immunosuppressive substance and widely used to prolong the survival preserve cells culture. objective this research was explore whether rapamycin supplementation during IVM enhances meiotic maturation, quality, subsequent embryonic development. Methods Depending on purpose, bovine cumulus complexes were without (control) or with at different concentrations (0, 1, 10, 100 nM) for 22 h (h) followed by 6 fertilization, zygotes cultured 8 days. Following treatment, oocytes assessed progression, intra-oocyte GSH synthesis, reactive oxygen species (ROS) levels, Results results showed that addition 1 nM medium increased nuclear (90.1 ± 1.5 vs. 81.2 2.5; P < 0.01) development (47.6 2.2 41.5 1.9; 0.05) observed control group. Consequently, compared group, relative fluorescence’s intensity ROS levels reduced treatment. More importantly, glutathione synthesis control, indicating bidirectional communication between becomes easy via Conclusions This study suggests presence can afford enhance synthesized oocyte, which internal antioxidant defense contributes improve high-quality embryo production.